Woolcock won the race in a time of 3h10min31sec, while Kleinhans clocked a time of 3h34min4sec.
This off-road race is annually held from Uniondale to Knysna via the Prince Alfred's Pass.
The event was organised by Zandile Meneses of Headstrong Productions and is owned by the Lions Clubs of Knysna and Uniondale.
All proceeds raised by the event are in aid of the Lions’ SightFirst for sight restoration operations for the poor.
The race started in Uniondale and riders tackled the notorious ‘ou wa pad’. The route then included the Prince Alfred's Pass hills and valleys before finishing in Knysna at the high school fields.

Pennypinchers Die Burger Karoo to Coast 100km Mountain Bike Challenge winners, Waylon Woolcock and Ariane Kleinhans on the podium. (Photo: Desmond Scholtz Photography)